Table 1.
Details of oligonucleotide used in the study along with product size and polymerase chain reaction conditions.
| Gene | NCBI Gene ID | RGD ID | Oligonucleotide Sequence |
GC% | Annealing Temperature | Product Size |
|---|---|---|---|---|---|---|
| MAFA | 366949 | 1562627 | Forward: GACCTGATGAAGTTCGAGGTG | 52.38 | 53.39 | 90 |
|
Reverse: GGGCGTCGAGGATAGCGA |
66.67 | 56.29 | ||||
| PDX-1 | 29535 | 62387 | Forward: CGTAGTAGCGGGACAACGAG | 60.00 | 55.25 | 71 |
| Reverse: CCCGAGGTTACGGCACAAT | 57.89 | 55.08 | ||||
| INS-1 | 16333 | 2915 | Forward: CTGGGAAATGAGGTGGAAAA | 45.00 | 56.65 | 98 |
|
Reverse: TCCACAAGCCACGCTTCTG |
57.89 | 56.89 | ||||
| INS-2 | 16334 | 2916 | Forward: AGAAAGGTTTGGTACCTGGAATAGAGC | 44.44 | 53.23 | 121 |
| Reverse: GTAGAAGAAGCTTCGCTCCCCACA | 54.16 | 53.76 | ||||
| NEUROG3 | 60329 | 631350 | Forward: CATAGCGGACCACAGCTTCT | 55.00 | 54.82 | 125 |
| Reverse: GGCTACCAGCTTGGGAAACT | 55.00 | 54.96 | ||||
| NKX6-1 | 65193 | 69318 | Forward: CGGAGAGTCAGGTCAAGGTC | 60.00 | 54.47 | 134 |
| Reverse: CTCGTTCTCCGAAGTCCCCT | 60.00 | 55.97 | ||||
| NEUROD | 29458 | 3165 | Forward: CATGTGTTCCACGTCAAGCC | 55.00 | 54.76 | 167 |
| Reverse: AACTCGGTGGATGGTTCGTG | 55.00 | 55.32 |