Table 2.

The contribution, and ANOVA of data of Box-Behnken matrix for lipase production on Nigella sativa by A. flavipes under semi-solid state fermentation conditions.

Source		Contribution, %	Degree of freedom	Sum of squares	Mean square	F-ratio	P-value
Overall model		98.95	9.00	22564.24	2507.14	52.13	0.00
Error	Lack of Fit	0.82	3.00	187.41	62.47	2.36	0.312*
	Pure	0.23	2.00	53.06	26.53
Single	X1	0.00	1.00	0.18	0.18	0.00	0.9536*
	X2	6.01	1.00	1370.00	1370.00	28.49	0.00
	X3	0.16	1.00	36.08	36.08	0.75	0.426*
Interaction	X1*X2	36.05	1.00	8221.05	8221.05	170.94	<.0001
	X1*X3	0.27	1.00	62.09	62.09	1.29	0.3074*
	X2*X3	5.52	1.00	1259.19	1259.19	26.18	0.00
Quadratic	X1*X1	11.96	1.00	3686.89	3686.89	76.66	0.00
	X2*X2	34.44	1.00	8251.01	8251.01	171.56	<.0001
	X3*X3	4.54	1.00	1034.33	1034.33	21.51	0.01
Model evaluation statistics
Determination coefficient (R2)				0.9895
Adjusted-R2				0.9705

X1; cultural humidity (ml water/g), X2; the surfactant; Brij 35 (μl/g), X3; inoculum density (spore/g).