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. 2023 Sep 22;14:1198310. doi: 10.3389/fimmu.2023.1198310

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Copyright © 2023 Sadeghalvad, Khijakadze, Orangi and Takei

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of ILC2s in the small intestine (SI) and liver of C57BL/6 mouse. (A) ILC2s in the SI were gated as Thy1⁺Lin⁻TCRs/CD3ϵ⁻ST2⁺GATA3⁺RORγt⁻, and RORγt⁺ILC3s (red square) were used as negative control. Histograms show the expression of ST2, KLRG1, and IL-17RB on ILC2s. (B) ILC2s in the liver were gated as Thy1⁺Lin⁻TCRs/CD3ϵ⁻GATA3⁺RORγt⁻. RORγt⁺ILC3s (red square) were used as negative control. Histograms show the expression of ST2, KLRG1, and IL-17RB. Gating was applied on live, single-cell, CD45⁺ leukocytes. Numbers indicate percentages of cells in each gate. (C) Frequency and number of ILC2s (of CD45⁺ leukocytes). Data are shown as mean ± SEM.