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. 2023 Sep 22;14:1198310. doi: 10.3389/fimmu.2023.1198310

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Copyright © 2023 Sadeghalvad, Khijakadze, Orangi and Takei

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The expression of Lin markers on RORγt⁺NK1.1⁻ cells (bold black square) in C57BL/6 mouse lung. Lin cocktail included TCR/CD3ϵ, CD11b, CD11c, GR-1, B220, and Ter119. Lin⁺ cells (red square) were used as positive control. Gating was applied on live, single-cell, CD45⁺ leukocytes. (B) The expression of RORγt on T cells. (C) ILC3s in C57BL/6 mouse lung were identified by serial gating as shown. (D) Expression of CD4, NKp46, and CCR6 on Rag1^−/− mouse lung ILC3s. Fluorescence minus one (FMO) was used as control negative. Gating applied on live, single, CD45⁺Lin⁻Thy1⁺NK1.1⁻ cells. Lin cocktail included Ter119, B220, CD11c, CD11b, and Gr-1. Numbers indicate percentages of cells in each gate.