Table 1.
Antibody list for flow cytometry.
| Antigen | Clone | Fluorochrome | Brand | Dilution |
|---|---|---|---|---|
| Viability dye | — | eFIuor780 | Thermo Fisher | 500 |
| CD45 | 30-F11 | BV510 | BD Biosciences | 100 |
| CD127 | A7R34 | PE | Thermo Fisher | 250 |
| CD127 | A7R34 | AF700 | Thermo Fisher | 80 |
| NKP46 | 29A1.4 | eFluor450 | Thermo Fisher | 250 |
| NKP46 | 29A1.4 | PerCPeF710 | Thermo Fisher | 200 |
| NKP46 | 29A1.4 | AF700 | Invitrogen | |
| CD19 | ID3 | eFluor450 | Thermo Fisher | 750 |
| LY6G and LY6C (GR-1) | R86-8C5 | eFluor450 | Thermo Fisher | 1000 |
| TCRβ | H57-597 | PE | BioLegend | 250 |
| TCRβ | H57-597 | FITC | BD Biosciences | 250 |
| TCRγδ | eBioGL3 | PE | BD Biosciences | 750 |
| TCRγδ | GL3 | FITC | BD Biosciences | 750 |
| CD3 | 145-2C11 | PE | BD Biosciences | 125 |
| CD3 | 145-2C11 | FITC | Thermo Fisher | 250 |
| GATA3 | TWAJ | APC | Thermo Fisher | 50 |
| Rorγt | B2D | PE | Thermo Fisher | 50 |
| Eomes | Dna11mag | PerCPeF710 | Thermo Fisher | 50 |
| T-bet | eBio4B10 | PECY7 | Thermo Fisher | 50 |
| Ter119 | TER119 | eFluor450 | Thermo Fisher | 250 |
| Thy1.2 | 33-2.1 | BV605 | BD Biosciences | 250 |
| B220 | RA3-6B2 | eFluor450 | Thermo Fisher | 750 |
| NK1.1 | PK136 | eFluor450 | Thermo Fisher | 250 |
| NK1.1 | PK136 | APC | BD Biosciences | 150 |
| CD11c | N418 | eFluor450 | Thermo Fisher | 750 |
| CD11b | M1/70 | eFluor450 | Thermo Fisher | 750 |
| ST2 | RMST2-2 | PECY7 | Thermo Fisher | 80 |
| CD49a | Ha31/8 | BV711 | BD Biosciences | 250 |
| CD49b | DX5 | AF647 | BioLegend | 250 |
| CCR6 | 140706 | BV711 | BD Biosciences | 250 |
| IFNγ | XMG1.2 | PECY7 | Thermo Fisher | 50 |
| IL-5 | TRFK5 | APC | BD Biosciences | 100 |
| IL-13 | eBio13A | PECY7 | Thermo Fisher | 50 |
| Rorγt | B2D | PE | Thermo Fisher | 50 |
| IL-17 | TC11-18H10 | PE | BD Biosciences | 50 |
| IL-22 | 1H8PWSR | PerCPeF710 | Thermo Fisher | 50 |