Table 2.
Antibody panel for ILC1, ILC2, and ILC3 identification.
| ILC1 panel | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| FITC | PerCPeF710 | AF647 | AF-700 | R780 | eFlour450 | BV510 | BV605 | BV650 | BV711 | PE | PECy7 |
| CD3ϵ, TCRβ, TCRγδ |
Eomes | CD49b | NK1.1, NKp46 | Viab | Ter119, Gr-1 CD19, B220 |
CD45 | Thy1.2 | CD160 | CD49a | CD127 | T-bet |
| ILC2 panel | |||||||||||
| FITC | PerCPeF710 | R670 | AF-700 | R780 | eFlour450 | BV510 | BV605 | BV650 | BV711 | PE | PECy7 |
| CD3ϵ, TCRβ, TCRγδ |
ST2 | GATA3 | – | Viab | Ter119, Gr-1 CD19, B220, NK1.1, NKp46 |
CD45 | Thy1.2 | – | – | Rorγt | CD127 |
| ILC3 panel | |||||||||||
| FITC | PerCPeF710 | R670 | AF-700 | R780 | eFlour450 | BV510 | BV605 | BV650 | BV711 | PE | PECy7 |
| CD3ϵ, TCRβ, TCRγδ |
ST2 | GATA3 | CD127 | Viab | Ter119, Gr-1 CD19, B220 |
CD45 | Thy1.2 | – | – | Rorγt | NK1.1 (or in Lin cocktail) |