Extended Data Fig. 5 ∣. AMD1 3’ UTR translation mitigation.

a, Western blot confirming the loss of the EGFP-AMD1 tail fusion protein. HEK293T cells were transfected with varying amount of the AMD1 3’ UTR readthrough reporter plasmid, from 50ng to 850ng. (N = 2 biologically independent samples). b, The AMD1 3’ UTR translation reporter with the hydrophobic region in the AMD1 tail highlighted (A-E). c, Impact of deleting individual hydrophobic regions or larger regions on the EGFP/mCherry ratio. The number in each plot is the median decrease of the EGFP/mCherry ratio relative to controls. d, BAG6 co-immunoprecipitates with EGFP:AMD1 fusion protein but not a mutated fusion protein with the functional hydrophobic region C-to-E deleted (AMD1^ΔH). N=4 biologically independent samples over 2 independent experiments for the quantification. Data are presented as mean values +/− s.d. P values calculated using two-sided Student’ t-test. No adjustments were made for multiple comparisons. ****: P < 0.0001.