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. Author manuscript; available in PMC: 2023 Oct 7.
Published in final edited form as: Nature. 2023 Apr 12;617(7960):395–402. doi: 10.1038/s41586-023-05946-4

Extended Data Fig. 6 ∣. Ribosome roadblock effect: comparing the AMD1 tail sequence, poly(A) and the XBP1 stalling sequence.

Extended Data Fig. 6 ∣

a-e, Reporter constructs shown on the left were transfected into HEK293T cells. The EGFP/mCherry ratio was quantified in individual cells using flow cytometry with distributions shown on the right on a log-10 scale. The number in each plot is the median fold-decrease of the EGFP/mCherry ratio. Note that AMD1 sequence causes less decrease in EGFP compared to both XBP1 and poly(A) sequences, and even this weak effect is independent of the putative pausing sequence in AMD1.