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. 2023 Oct 7;14:6262. doi: 10.1038/s41467-023-41824-3

Fig. 5. Exogenous gibberellin application can rescue the dwarf phenotype of Gmilpa1-2.

Fig. 5

a Phenotypes of Hedou 12 (H12) and Gmilpa1-2 seedlings grown under sunlight treated with GA3 at the V1 stage (one unrolled trifoliate leaf), Scale bar, 20 cm. b Plant height of the seedlings at the V4 stage (three unrolled trifoliate leaves) in (a), (n = 10 biologically independent samples; P = 1.92 × 10−14 under mock condition). c Phenotypes of H12, Gmilpa1-2, and Gmilpa1 seedlings treated with PAC alone and PAC with GA3 treated with UV-B for 14 days, scale bar, 10 cm. d Plant height of the seedlings in (c), (n = 7 biologically independent samples; P = 1.12 × 10−5 under UV-B condition). e–j Endogenous GAs levels in H12 and Gmilpa1-2. GA levels were measured using apical buds of V2-stage (two unrolled trifoliate leaves) seedlings grown under white light and white light with UV-B, (n ≥ 3 biologically independent samples; P = 6 × 10−4 in e P = 0.0134 in f P = 6.90 × 10−6 in g P = 3.71 × 10−7 in h P = 1.29 × 10−7 in i P = 4 × 10−4 in i). P-values were calculated compared to H12 seedlings by two-tailed Student’s t-tests; ***P < 0.001, *P < 0.05; ns not significant. k Immunoblots showing GmILPA1 abundance in seedlings of H12 at the V1 stage (unrolled one trifoliate leaf) treated with 100 μM GA3 and UV-B for the indicated times. l Phenotypes of H12, Gmilpa1-2, and T2 progeny lines of Gmilpa1-2/GmGA2ox-like RNAi grown under white light with UV-B (600 μmol m−2 s−1, 1.5 μmol m−2 s−1 UV-B, 12-h light/12-h dark). Scale bar, 10 cm. m Plant height of the seedlings, (n = 4 biologically independent samples for H12 and Gmilpa1-2, n = 6 biologically independent samples for Gmilpa1-2/GmGA2ox-like-RNAi, P = 1.01 × 10−4 in H12 and Gmilpa1-2, P = 5.21 × 10−4 in H12 and Gmilpa1-2/GmGA2ox-like-RNAi, P = 9.29 × 10−5 in Gmilpa1-2 and Gmilpa1-2/GmGA2ox-like-RNAi). In b, d, m, Data are presented as mean values ± SD, P-value is calculated with a one-way ANOVA analysis–Tukey comparison, and the columns labeled without the same alphabet are significantly different (P < 0.05, two-sided). ***P < 0.001, ns, not significant. Source data are provided as a Source Data file.