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. 2023 Sep 15;10:rbad085. doi: 10.1093/rb/rbad085

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of tFNAs on the activity of the PI3K/Akt signalling pathway of hUMSCs. (A) Protein expression of p-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR in hUMSCs treated with tFNAs. (B) Quantitative analysis of the protein expression levels of p-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR. Data are presented as the mean ± SD (n = 3). (C–E) Immunofluorescence detection of p-PI3K, p-Akt and p-mTOR (cytoskeleton: green, nucleus: blue and protein: red). Scale bars are 25 μm. (F) Quantitative analysis of the average optical density of fluorescence images. Statistical analysis: *P < 0.05, **P < 0.01, ***P < 0.001.