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. Author manuscript; available in PMC: 2023 Oct 8.
Published in final edited form as: ACS Chem Biol. 2023 Aug 21;18(9):2030–2038. doi: 10.1021/acschembio.3c00257

Figure 5: Biochemical validation of activating mutants.

Figure 5:

a) Bar plot showing fold-change in DNMT3A methyltransferase activity of mutants vs WT, based on data in b). P-values were calculated by unpaired two-tailed students’ t-tests. Fold-change errors were propagated from the individual standard deviations.

b) Bar plot showing methyltransferase activity of DNMT3A mutants ± 5 μM H3K4me0. Data are mean ± SD of n = 3 replicates. P-values were calculated by unpaired two-tailed students’ t-tests. See Figure S7 for SDS-PAGE gel of purified proteins and replicate 2.