Figure 1. Organ baths to assess motility in (A) small intestine (SI) and colon preparations and (B) caecum.

(A) The colon/small intestinal organ bath has two chambers enabling two tissue preparations to be assayed simultaneously. (B) The caecum organ bath is a Petri dish that allows for the three-way cannulation of the caecum preparation. The temperature of setup (A) is regulated by an external water bath. The caecum setup (B) is placed on a heat block to ensure the temperature is kept constant throughout video recording. White arrows indicate the flow of Krebs solution and therefore the pressure exerted on the gut preparations.