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. 2023 Oct 5;13(19):e4831. doi: 10.21769/BioProtoc.4831

Figure 4. GutMap contraction wave annotation windows.

Figure 4.

(A) In the Heatmap Analysis Window, lock the colour range and select Annotate Contraction Waves. A new window will open, allowing the user to manually annotate the contractions or groups of contractions. Annotated examples are shown for (B) colonic, (C) jejunal, and (D) caecal heatmaps [annotated region depicts caecal contractions propagating from the colon-caecal junction (upper red region of map) towards the caecal tip]. The Contraction Wave Annotation tool can be used to measure velocity, duration, and length of the contraction by selecting Manually Annotate and drawing a line following the slope of the contraction or contractile cluster. The data obtained in the results table can be exported by selecting Export Data in the Heatmap Analysis Window.