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SDS-PAGE of recombinant Y49 β-lactamase. The β-lactamase-containing fractions from nickel affinity chromatography were pooled, and 1 μg of protein was loaded onto an SDS–12% polyacrylamide gel. Lane 1, molecular mass markers; lane 2, purified recombinant Y49 β-lactamase. The six-histidine tag adds approximately 3 kDa to the mass of the recombinant Y49 β-lactamase.
