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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Apr 9;42(4):112368. doi: 10.1016/j.celrep.2023.112368

Figure 3. Characterization of protein N-glycosylation by MS/MS.

Figure 3.

(A) Illustration of the N-glycan maturation sequence for the predominant brain N-glycans. Many potential glycans contain 0–3 fucoses, with some examples shown here.

(B) Illustrative annotated AI-ETD spectrum with glycopeptide precursor shown. Curved lines annotated with glycan compositions denote Y ions, which contain the intact peptide and a fragmented glycan. Both b/y and c/z ions are detected owing to hybrid vibrational and electron transfer modes of fragmentation.

(C) As in (B) but with sceHCD fragmentation of the depicted glycopeptide, which yields b/y and Y ions.