Figure 6. Deep glycoproteomics of the SV reveals site-specific biases toward mannosylation or fucosylation of SV proteins.

Several of the most abundant SV glycoproteins are depicted with AlphaFold structures⁶⁵ and filled circles corresponding to unique N-glycopeptides detected for each N-linked glycosylation site. Sites are noted by the amino acid sequence number for each glycosylated asparagine. High fucosylation was observed for all SV glycoproteins shown here except for syt1. SV glycoproteins with multiple glycosylation sites demonstrated a bias toward fucosylation at a single site, while other sites were more likely to bear oligomannose or other non-fucosylated N-glycans. No glycoPSMs were observed for peptides containing N548 on SV2A.

See also Table S6, which contains the glycoPSM data used to generate this figure, and Figure S6, which addresses prior studies on the glycosylation of SV2.