KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| SV2 mAb | DSHB | SV2 (RRID:AB_2315387) |
| Syt1 mAb | DSHB | mAb 48 (RRID:AB_2199314) |
| Guinea pig anti-synaptophysin | Synaptic Systems | 104 211 (RRID:AB_1210382) |
| Goat anti-mouse HRP-conjugated secondary Ab | Bio-Rad | 1706516 |
| Goat anti-guinea pig HRP-conjugated secondary Ab | Thermo Fisher | A18769 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Protein G Sepharose fast flow | Cytiva | 17061801 |
| Dynabeads M-270 epoxy | Thermo Fisher | 14302D |
| cOmplete mini, EDTA-free | Sigma (Roche) | 04693159001 |
| Dynabeads M-270 carboxylic acid | Thermo Fisher | 14305D |
| Trypsin | Promega | V5111 |
| SOLA SAX 10 mg cartridge | Thermo Fisher | 60109–003 |
| PNGase F | NEB | P0708 |
| Procainamide | Sigma | SML2088 |
| Sodium cyanoborohydride, 5 M in 1 M NaOH | Sigma | 296945 |
| OASIS HLB 30 mg cartridge | Waters | WAT094225 |
| α(1–2,3,6) mannosidase | NEB | P0768 |
| α(1–3,4) fucosidase | NEB | P0769 |
| α(1–2) fucosidase | NEB | P0724 |
| α(1–2,4,6) fucosidase O | NEB | P0749 |
| β(1–3,4) galactosidase | NEB | P0746 |
| β-GlcNAcase S | NEB | P0744 |
|
| ||
| Deposited data | ||
|
| ||
| LC-MS raw data deposited at MassIVE | This paper | https://doi.org/10.25345/C5TB0Z526 |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| Mouse: C57B6/J | Jackson Laboratory | 000664 (RRID:IMSR_JAX:000664) |
|
| ||
| Software and algorithms | ||
|
| ||
| FragPipe | Nesvizhskii Lab | v. 17.1 |
| Agilent ChemStation | Agilent | LTS 01.11 |
| R/Rstudio | CRAN/Posit | v. 2021.09.0 |
| R scripts used in this paper | This paper | https://doi.org/10.5281/zenodo.7659070 |
| The Gene Ontology resource | Geneontology.org | v. 2023–01-01 |
| Prism | GraphPad | v. 9.3.1 |