KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-LDH-A (C4B5) Rabbit mAb | Cell Signaling Technology | Cat# 3582; RRID: AB_2066887 |
| Anti-COX IV Mouse mAb | TransGen Biotech | Cat# HL101-01; N/A |
| Anti-Glut1 (D3J3A) Rabbit mAb | Cell Signaling Technology | Cat# 12939; RRID: AB_2687899 |
| Anti-VDAC1 Rabbit mAb | Abways Technology | Cat# CY5416; RRID: AB_2773723 |
| Anti-Cytochrome C pAb | Proteintech | Cat# 10993-1-AP; RRID: AB_2090467 |
| Anti-Hsp60 (A57-B9) Mouse mAb | Santa Cruz Biotechnology | Cat# SC-57840; RRID: AB_783868 |
| Anti-Flag mAb | MBL | Cat# M185-7;RRID: AB_2687989 |
|
Bacterial and virus strains | ||
| BL21(DE3) Chemically Competent Cell | TransGen Biotech | Cat# CD601-02 |
| Trans5α Chemically Competent Cell | TransGen Biotech | Cat# CD201-02 |
|
Biological samples | ||
| Human-derived Clinical Sample | Shanghai Jiao Tong University affiliated Sixth People’ s Hospital; Shanghai East Hospital | N/A |
|
Chemicals, peptides, and recombinant proteins | ||
| 3-Bromopyruvic Acid (3-BrPA) | Alfa Aesar | Cat# L00720 |
| Sodium Oxamate | Sigma-Aldrich | Cat# O2751 |
| Rotenone | Sigma-Aldrich | Cat# R8875 |
| Oligomycin A | MedChemExpress | Cat# HY-16589 |
| CCCP | MedChemExpress | Cat# HY-100941 |
| Antimycin A | Sigma-Aldrich | Cat# A8674 |
| AZD-3965 | TargetMol | Cat# T3210 |
| AR-C155858 | MedChemExpress | Cat# HY-13248 |
| SB-204990 | MedChemExpress | Cat# HY-16450 |
| BMS-303141 | APExBIO | Cat# B1079 |
| Rapamycin | Shanghai YEASEN | Cat# 52404ES60 |
| Octyl-(R)-2HG | Sigma-Aldrich | Cat# SML2200 |
| Octyl-(S)-2HG | J&W Pharmlab | Cat# 05R0850 |
| Ionomycin | Aladdin | Cat# I139530 |
| Compound 968 | MedChemExpress | Cat# HY-12682 |
| CB-839 | MedChemExpress | Cat# HY-12248 |
| β-Nicotinamide Mononucleotide (NMN) | Shanghai YEASEN | Cat# 60303ES80 |
| Nicotinamide Riboside (NR) | Shanghai Yuanye Bio-technology | Cat# S24896 |
| Nicotinamide (NAM) | Sigma-Aldrich | Cat# N3376 |
| Nicotinic Acid (NA) | Sigma-Aldrich | Cat# N4126 |
| FK866 Hydrochloride Hydrate | Sigma-Aldrich | Cat# F8557 |
| STF-118804 | Selleck | Cat# S7316 |
| Coenzyme Q10 (CoQ) | Sigma-Aldrich | Cat# C9538 |
| MitoQ10 Mesylate (MitoQ) | MedChemExpress | Cat# HY-100116A |
| Troglitazone | MedChemExpress | Cat# HY-50935 |
| Metformin | Ourchem | Cat# XW11157041 |
| Hieff Trans Liposomal Transfection Reagent | Shanghai YEASEN | Cat# 40802ES03 |
| DMEM Media (High Glucose) | HyClone | Cat# SH30243.01 |
| RPMI Medium Modified (1640) | HyClone | Cat# SH30809.01B |
| PBS Powder | Absin | Cat# abs9266 |
| Opti-MEM | Gibco | Cat# 31985-070 |
| Fetal Bovine Serum (FBS) | Biological Industries | Cat# 04-001-1ACS |
| Trypsin-EDTA | Gibco | Cat# 25200-072 |
| Human Fibronectin | Shanghai YEASEN | Cat# 40105ES08 |
| Streptozotocin | Sigma-Aldrich | Cat# S0130 |
| Insulin | Sigma-Aldrich | Cat# I9278 |
| Catalase | Sigma-Aldrich | Cat# C3155 |
| Sodium Pentobarbital | Sigma-Aldrich | Cat# P3761 |
| Sodium L-lactate | Sigma-Aldrich | Cat# 71718 |
| Polybrene | Shanghai YEASEN | Cat# 40804ES76 |
| Puromycin | Shanghai YEASEN | Cat# 60210ES25 |
| NAD+ | Shanghai YEASEN | Cat# 60323ES08 |
| NADH | Shanghai YEASEN | Cat# 60301ES03 |
| NADP+ | Shanghai YEASEN | Cat# 60324ES03 |
| NADPH | Shanghai YEASEN | Cat# 60302ES01 |
| ADP | Shanghai YEASEN | Cat# 60604ES03 |
| ATP | Sigma-Aldrich | Cat# A26209 |
| D-(+)-Glucose | Macklin | Cat# D810594 |
| Phosphoenolpyruvic Acid | Alfa Aesar | Cat# B20358 |
| Sodium Pyruvate | Aladdin | Cat# S104174 |
| Oxaloacetic Acid | Sigma-Aldrich | Cat# O4126 |
| Sodium Citrate | Aladdin | Cat# S189183 |
| Isocitric Acid | Sigma-Aldrich | Cat# I1252 |
| α-Ketoglutaric Acid | Sigma-Aldrich | Cat# 75892 |
| Succinic Acid | Aladdin | Cat# S108855 |
| Fumaric Acid | Aladdin | Cat# F118459 |
| L-Malic Acid | Sigma-Aldrich | Cat# 112577 |
| β-Hydroxybutyrate | MedChemExpress | Cat# HY-113378 |
| Sodium Acetate | Titan | Cat# G18900B |
| Glycine | Titan | Cat# G65953B |
| L-Isoleucine | Macklin | Cat# L811667 |
| L-Threonine | Macklin | Cat# T6272 |
| L-Glutamine | Macklin | Cat# L810391 |
| L-Alanine | Macklin | Cat# L800640 |
| L-Aspartic Acid | Macklin | Cat# A6202 |
| L-Histidine | Macklin | Cat# 811075 |
| L-Tryptophan | Macklin | Cat# L818799 |
| L-Glutamic Acid | Macklin | Cat# L810368 |
| L-Methionine | Macklin | Cat# L812760 |
| L-Serine | Macklin | Cat# S6199 |
| L-Proline | Macklin | Cat# L816039 |
| L-Lysine | Macklin | Cat# L812314 |
| L-Asparagine | Macklin | Cat# L800456 |
| L-Leucine | Macklin | Cat# L6238 |
| L-Phenylalanine | Macklin | Cat# L816180 |
| L-Arginine | Macklin | Cat# A6286 |
| L-Valine | Macklin | Cat# L820396 |
| L-Tyrosine | Macklin | Cat# T6229 |
| L-Cystenine | Macklin | Cat# L804954 |
| Dithionitrobenzoic acid (DNTB) | Alfa Aesar | Cat# A14331 |
| Acetyl-CoA | Shanghai Yuanye Bio-technology | Cat# S10019 |
| Sucrose | Energy Chemical | Cat# D100088 |
| D-Mannitol | Sigma-Aldrich | Cat# M4125 |
| EGTA | Sigma-Aldrich | Cat# E3889 |
| EDTA | GENERAL-REAGENT | Cat# G68915B |
| Resazurin | Macklin | Cat# R817239 |
| Diaphorase | Sigma-Aldrich | Cat# D5540 |
| L-Lactate Dehydrogenase | Sigma-Aldrich | Cat# L2625 |
| Digitonin | Calbiochem | Cat# 300410 |
| Low Melting Point Agarose | Shanghai YEASEN | Cat# 10214ES08 |
| MitoTracker Red | Thermo Scientific | Cat# M22425 |
| JC-10 | Shanghai YEASEN | Cat# 40707ES03 |
| 2×Hieff™ PCR Master Mix | Shanghai YEASEN | Cat# 10102ES08 |
| PrimeSTAR® Max DNA Polymerase | Takara | Cat# R045B |
| 2×Pfu PCR MasterMix | TIANGEN | Cat# KP201-02 |
| T4 Polynucleotide Kinase | Thermo Scientific | Cat# EK0032 |
| T4 DNA Ligase | Thermo Scientific | Cat# EL0012 |
| FastDigest EcoRI | Thermo Scientific | Cat# FD0274 |
| FastDigest NotI | Thermo Scientific | Cat# FD0594 |
| Super ECL Detection Reagent | Shanghai YEASEN | Cat# 36208ES60 |
| Lenti-Pac Lentivirus Concentration Solution | GeneCopoeia | Cat# LT007 |
| Protease/Phosphatase Inhibitor Cocktail | Bimake | Cat# B14001 |
|
Critical commercial assays | ||
| Cell Mitochondria Isolation Kit | Beyotime Biotechnology | Cat# C3601 |
| BCA Protein Quantification Kit | Shanghai YEASEN | Cat# 20201ES76 |
| Hieff Clone Plus One Step Cloning Kit | Shanghai YEASEN | Cat# 10911ES20 |
| PicoProbeTM Lactate Fluorometric Assay Kit | BioVision | Cat# K638 |
| Angilent Seahorse XFe96 Extracellular Flux Assay Kit | Agilent | Cat# 102418-00013 |
|
Experimental models: cell lines | ||
| HEK293T | Cell Bank of Chinese Academy of Science | GNHu17 |
| HEK293 | Cell Bank of Chinese Academy of Science | GNHu43 |
| NCI-H1299 | Cell Bank of Chinese Academy of Science | TCHu160 |
| HeLa | Cell Bank of Chinese Academy of Science | TCHu187 |
|
Experimental models: organisms/strains | ||
| C57BL/6J Mice | East China Normal University | N/A |
| Type I Diabetic Mice (T1DM) | East China Normal University | N/A |
| The db/db Mice (T2DM) | East China Normal University | N/A |
|
Oligonucleotides | ||
| Primer sequences for cloning or sequencing | This paper | See Table S1 |
|
Recombinant DNA | ||
| pAAV-CMV-MCS-FiLa | This paper | N/A |
| pAAV-CMV-MCS-FiLa-C | This paper | N/A |
| pcDNA3.1-Nuc-FiLa | This paper | N/A |
| pcDNA3.1-Nuc-FiLa-C | This paper | N/A |
| pcDNA3.1-Mit-FiLa | This paper | N/A |
| pcDNA3.1-Mit-FiLa-C | This paper | N/A |
| pLVX-FiLa | This paper | N/A |
| pLVX-FiLa-C | This paper | N/A |
| pLVX-Nuc-FiLa | This paper | N/A |
| pLVX-Nuc-FiLa-C | This paper | N/A |
| pLVX-Mit-FiLa | This paper | N/A |
| pLVX-Mit-FiLa-C | This paper | N/A |
| pLVX-NES-FiLa | This paper | N/A |
| pLVX-NES-FiLa-C | This paper | N/A |
| pLVX-Nuc-SoNar | Zhao et al., 2015 | N/A |
| pLVX-Nuc-iNapc | Tao et al., 2017 | N/A |
| pLVX-NES-SoNar | Zhao et al., 2015 | N/A |
| pLVX-NES-iNapc | Tao et al., 2017 | N/A |
| pLVX-Mit-Frex | Zhao et al., 2011 | N/A |
| pLVX-Mit-cpYFP | This paper | N/A |
| pLVX-Mit-TagRFP | This paper | N/A |
| pCDFDuet-FiLa | This paper | N/A |
| pCDFDuet-FiLa-H | This paper | N/A |
| pCDFDuet-FiLa-C | This paper | N/A |
| pCDFDuet-LOX | This paper | N/A |
| psPAX2 | Addgene | Cat# 12260 |
| pMD2.G | Addgene | Cat# 12259 |
|
Software and algorithms | ||
| ImageJ 1.50d | Fiji | http://fiji.sc/ |
| GraphPad Prism V8.0.2.263 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Gen5 3.03 | Biotek | https://www.biotek.com/products/software-robotics/ |
| Leica Application Suite X 1.8.1.13759 | Leica | https://www.leica-microsystems.com.cn/cn/products/microscope-software/p/leica-application-suite/ |
| SnapGene 2.0.0 | GSL Biotech | https://download.cnet.com/s/snapgene/ |
| CytExpert2.3 | Beckman Coulter | https://www.mybeckman.cn/flow-cytometry/research-flow-cytometers/cytoflex/software |
| Wave Desktop | Agilent | https://www.agilent.com.cn/zh-cn/products/cell-analysis/software-download-for-wave-desktop |
|
Other | ||
| 35 mm 4-chamber Glass Bottom Dish | Cellvis | D35C4-20-1-N |
| 8 Chambered Coverglass System | Cellvis | C8-1.5H-N |