Figure 4.
Activation of ER stress in chemoresistant cancer cells under the treatments of endocannabinoids and chemotherapeutic drugs. A-D. Cisplatin-resistant ES2 and IGROV1 cell lines were treated with 2 mM cisplatin and IC30 of AEA and 2-AG for 12, 24 and 48 h. Antibodies against ER stress-related proteins, including Grp78, ATF6α, phospho-IRE1α (p-IRE1α), IRE1α, phospho-eIF2α (p-eIF2α) and eIF2α were used in the immunoblotting analysis. 10 μg/mL tunicamycin was served as positive control (+) of ER stress. β-actin was served as the internal control. FL: full-length form, CL: cleaved form.