Figure 6.
Endocannabinoids combined with chemotherapeutic drugs induce apoptosis in chemoresistant cancer cells. A. Chemoresistant cancer cells were treated with 2 mM cisplatin, 0.01 μg/mL (for ES2-TX) or 0.1 μg/mL (for IGROV1-TX) paclitaxel and IC30 of AEA or 2-AG for 48 h. Annexin V and propidium iodide were used to quantify apoptosis. Representative images of flow cytometry were shown. B-E. Quantification of the percentage of apoptotic cells was analyzed from three independent experiments. The bars represent mean ± SEM. F. Schematic diagram shows the mechanism about how endocannabinoids reverse chemoresistance in OVC. *P<0.05, **P<0.01, ***P<0.001 by one-way ANOVA.