Figure 6.

PBMC innate effector cytokine/chemokine expression in responses to bacterial, fungal, and viral PAMPs increases after BCG revaccination

PBMC at T0 and T2 from N = 13 (male = 6, female = 7) vaccinees were stimulated or not with Candida albicans, LPS, Pam-3-CSK, and poly I:C and culture supernatants were collected after 24 h. TNF-α, IL-1β, IL-6, CXCL-10, IFN- α, IFNγ, CXCL-9, GM-CSF, and IL-12p70 were measured and analyte concentrations after matched unstimulated background subtraction were plotted as heat-maps. Unstimulated control for all mediators at T0 and T2 are shown as a separate heatmap. p values for differences between T0 and T2 are shown next to the heat-maps and significant differences of p < 0.05 are highlighted by blue stars. Wilcoxon matched-pairs signed rank test was used to determine p values which are shown for each comparison. p < 0.05 was considered significant.