Feeding neurons integrate metabolic and reproductive states in mice

. 2023 Sep 14;26(10):107918. doi: 10.1016/j.isci.2023.107918

List of viral vectors used

Experiment	Virus	Depositor & procurement	Titer (vg/mL)	Volume (nL)	Citation
Caspase ablation	AAV2-flex-taCasp3-TEVp	Nirao Shah & Jim Wells, UNC Vector Core	1–8 x 10¹²	200–250	Yang et al.⁵⁸
Transient activation	AAV8-hSyn-hM3D(Gq)-mCherry	Brian Roth, Addgene viral prep # 50474-AAV8	≥4 × 10¹²	150–200	Krashes et al.⁵⁷
Fluorescent localization	AAV2-FLEX-tdTomato	Edward Boyden, Addgene plasmid #28306	≥5 × 10¹²	200 for Flow-Seq; 400 for Esr1-Cre
Fluorescent controls	AAV8-Syn-FLEX-Mac-GFP	Edward Boyden, Addgene plasmid #58852	1:5 dilution of stock	Matching volume to experimental animals