Table 1.
Hepatic and serum ceramide content of ethanol (EtOH)-fed ceramide synthase 6 (CerS6) knockout and wild-type mice.
| Cer species | Liver ceramides (pmol/mg protein) |
p-value | Serum ceramides (nM) |
p-value | ||
|---|---|---|---|---|---|---|
| WT | KO | WT | KO | |||
| Male | ||||||
| C14:0 | 1.90 ± 0.29 | 0.59 ± 0.08 | 0.00 | 8.47 ± 1.78 | 6.27 ± 0.70 | 0.20 |
| C16:0 | 42.90 ± 4.01 | 30.49 ± 2.88 | 0.03 | 63.16 ± 10.93 | 11.84 ± 1.38 | 0.00 |
| C18:0 | 24.59 ± 3.24 | 16.62 ± 2.04 | 0.05 | 79.28 ± 16.99 | 47.06 ± 19.96 | 0.30 |
| C18:1 | 5.06 ± 0.41 | 2.61 ± 0.33 | 0.00 | 8.75 ± 1.87 | 5.45 ± 1.60 | 0.22 |
| C20:0 | 109.07 ± 13.42 | 84.93 ± 12.56 | 0.24 | 526.29 ± 81.39 | 136.31 ± 23.88 | 0.00 |
| C20:1 | 12.53 ± 2.07 | 6.07 ± 0.80 | 0.01 | 28.23 ± 5.01 | 10.59 ± 2.12 | 0.00 |
| C20:4 | 0.60 ± 0.19 | 0.21 ± 0.06 | 0.04 | 1.44 ± 0.50 | 0.26 ± 0.08 | 0.01 |
| C22:0 | 120.26 ± 10.55 | 187.93 ± 21.59 | 0.05 | 1617.95 ± 280.61 | 991.36 ± 185.34 | 0.08 |
| C22:1 | 63.53 ± 4.78 | 74.43 ± 6.81 | 0.29 | 449.24 ± 54.08 | 193.21 ± 42.79 | 0.00 |
| C24:0 | 87.34 ± 8.24 | 140.21 ± 15.56 | 0.03 | 1352.47 ± 249.49 | 916.71 ± 146.07 | 0.13 |
| C24:1 | 102.17 ± 11.93 | 203.30 ± 25.43 | 0.02 | 1120.24 ± 187.58 | 629.39 ± 110.88 | 0.03 |
| C26:0 | 0.20 ± 0.05 | 0.43 ± 0.08 | 0.07 | 10.61 ± 2.11 | 4.43 ± 0.81 | 0.01 |
| C26:1 | 0.32 ± 0.03 | 0.50 ± 0.09 | 0.11 | 9.01 ± 1.49 | 4.99 ± 0.69 | 0.02 |
| Female | ||||||
| C14:0 | 2.02 ± 0.06 | 0.57 ± 0.04 | 0.00 | 4.87 ± 0.72 | 2.02 ± 0.17 | 0.01 |
| C16:0 | 45.63 ± 4.14 | 7.54 ± 0.54 | 0.00 | 106.98 ± 7.65 | 15.36 ± 1.87 | 0.00 |
| C18:0 | 15.88 ± 0.75 | 15.01 ± 1.28 | 0.58 | 36.72 ± 5.55 | 31.65 ± 2.75 | 0.44 |
| C18:1 | 1.94 ± 0.14 | 1.60 ± 0.10 | 0.08 | 4.26 ± 0.32 | 2.75 ± 0.27 | 0.01 |
| C20:0 | 26.92 ± 1.85 | 28.44 ± 2.56 | 0.64 | 62.35 ± 9.43 | 50.24 ± 4.45 | 0.28 |
| C20:1 | 3.49 ± 0.26 | 2.77 ± 0.28 | 0.10 | 3.64 ± 0.39 | 3.33 ± 0.76 | 0.72 |
| C20:4 | 0.19 ± 0.02 | 0.20 ± 0.06 | 0.78 | ⁻ | ⁻ | ⁻ |
| C22:0 | 35.88 ± 2.78 | 44.53 ± 2.21 | 0.04 | 223.50 ± 19.00 | 254.14 ± 15.08 | 0.24 |
| C22:1 | 23.46 ± 1.09 | 23.78 ± 2.35 | 0.91 | 73.70 ± 6.12 | 89.72 ± 7.37 | 0.13 |
| C24:0 | 115.02 ± 8.26 | 181.84 ± 12.80 | 0.00 | 475.89 ± 25.99 | 976.01 ± 57.35 | 0.00 |
| C24:1 | 86.63 ± 4.53 | 100.04 ± 9.30 | 0.23 | 485.18 ± 24.27 | 510.14 ± 45.51 | 0.64 |
| C26:0 | 2.83 ± 0.28 | 3.79 ± 0.34 | 0.06 | 14.08 ± 1.70 | 29.50 ± 1.45 | 0.00 |
| C26:1 | 1.67 ± 0.13 | 2.67 ± 0.08 | 0.00 | 9.13 ± 0.68 | 16.39 ± 1.76 | 0.01 |
Data are reported as mean ± SEM; n = 5–9/group.
A two-tailed unpaired t-test was used to compare WT and CerS6 KO mice.