Figure 1. LmOVA infection prior to pregnancy induces the accumulation of CD4⁺ and CD8⁺ T cells in the uterus.

• A
  Experimental approach.
• B
  Numbers of CD4⁺ and CD8⁺ in 1 × 10⁶ uterine cells on gd 18.5 after preconceptual infection of the mother in comparison with non‐infected mothers (n = 7, n = 9, n: biological replicates). T cells were identified as CD45⁺CD3⁺ and either CD4⁺ or CD8⁺ cells.
• C
  Representative dot plots of CD8⁺ T cells from peripheral blood divided in CD44⁺CD62L⁻ effector/effector memory (EM), CD44⁺CD62L⁺ central memory (CM), and CD44⁻CD62L⁺ naïve subpopulations. Left: non‐infected pregnant control mouse, right: previously infected pregnant mouse.
• D–G
  Percentage of EM T cells (CD44⁺, CD62L⁻) among CD8⁺ T cells; (D) peripheral blood (n = 6, n = 9), (E) uterus‐draining lymph nodes (n = 6, n = 9), (F) spleen (n = 7, n = 8), (G) uterus (n = 7 each); n: biological replicates.
• H
  Representative dot plots of ovalbumin‐specific CD8⁺ EM T cells stained with H‐2K^b ovalbumin_257‐264 tetramers (OVA tetramers) from peripheral blood. Left: non‐infected pregnant control mouse, right: previously infected pregnant mouse.
• I–L
  Percentage of ovalbumin‐specific CD8⁺ EM T cells; (I) peripheral blood (n = 7, n = 9), (J) uterus‐draining lymph nodes (n = 7, n = 9), (K) spleen (n = 8 each), (L) uterus (n = 6, n = 9); n: biological replicates.

Data information: In (B), (D–G), (I–L), data are presented as mean ± SEM. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001 (E, J, K, L: Student's t‐test; B, D, F, G, I: Mann–Whitney‐U test).

Source data are available online for this figure.