Table 1. Chemical Unfolding of Lysozyme with Guanidine HCl at Different Temperatures.
| temp | cmid | KD | cini | cend | ΔF | gden |  |
m | |
|---|---|---|---|---|---|---|---|---|---|
| °C | M | M–1 | σ | M | M | kcal/mol | kcal/mol | kcal/mol | kcal L/mol2 |
| 1020 | 3.8 | 0.263 | 1.50 × 10–03 | 2.7 | 5.3 | –21.8 | –0.379 | 9.55 | 2.512 |
| 1520 | 4.0 | 0.25 | 1.80 × 10–03 | 2.7 | 5.6 | –22.7 | –0.417 | 8.598 | 2.150 |
| 2020 | 3.9 | 0.257 | 2.00 × 10–03 | 2.6 | 5.5 | –23.8 | –0.436 | 8.598 | 2.210 |
| 2520 | 3.8 | 0.263 | 2.50 × 10–03 | 2.4 | 5.5 | –26.1 | –0.491 | 9.554 | 2.513 |
| 3520 | 3.3 | 0.303 | 5.00 × 10–03 | 1.75 | 5.1 | –32.6 | –0.654 | 8.598 | 2.605 |
| 1017 | 3.8 | 0.26 | 1.00 × 10–03 | 2.9 | 5.25 | –20.1 | –0.334 | 9.55 | 2.483 |
| 4017 | 2.9 | 0.34 | 2.50 × 10–03 | 1.9 | 4.1 | –26.41 | –0.493 | 8.36 | 2.842 |
| 6017 | 1.1 | 0.9 | 3.00 × 10–02 | 0.25 | 2.4 | –65.5 | –1.5 | 4.299 | 3.869 |
| 2522 | 3.9 | 0.256 | 1.00 × 10–03 | 2.8 | 5.15 | –21.2 | –0.361 | 10.03 | 2.568 |
| 1021 | 4.1 | 0.243 | 2.00 × 10–03 | 2.7 | 5.8 | –22.55 | –0.43 | 8.837 | 2.147391 |
| 3015 | 3.9 | 0.258 | 1.00 × 10–03 | 2.9 | 5.3 | –21.7 | –0.363 | 8.837 | 2.236 |
| 4515 | 3.2 | 0.31 | 1.00 × 10–02 | 1.3 | 5.3 | –39.3 | –0.888 | 4.777 | 2.236 |