Table 2. Chemical Unfolding of Peptides and Proteins of Different Sizes and Structures in Guanidine HCl and Urea.
| protein, peptide | Naa | pH | denaturant | KD | cmid | σ | cini | cend | Δc | gden | ΔF | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M–1 | M | M | M | M | kcal/mol | kcal/mol | lit. | figure | |||||
| BBL | 41 | 7 | GndHCl | 0.33 | 3.03 | 3.00 × 10–02 | 0.2 | 7.5 | 7.3 | –2.14 | –20.9 | (31) | Figure 2B |
| ubiquitin WT | 76 | GndHCl | 0.34 | 2.94 | 7.00 × 10–03 | 1.4 | 5 | 3.6 | –0.753 | –22.1 | (32) | Figure 1 | |
| ubiquitin L67S | 76 | GndHCl | 0.72 | 1.39 | 1.30 × 10–02 | 0.5 | 2.7 | 2.2 | –0.998 | –28.4 | (32) | Figure 1 | |
| ubiquitin | 76 | 2 | GnddHCl | 0.395 | 2.53 | 8.00 × 10–03 | 1.2 | 4.5 | 3.3 | –0.782 | –24.1 | (33) | Figure 4 |
| ubiquitin | 76 | 5.5 | GndHCl | 0.295 | 3.39 | 1.20 × 10–02 | 1.3 | 6.3 | 5 | –0.934 | –26.4 | (33) | |
| Apo lipoprotein A1 | 120 | 8 | GndHCl | 1 | 1.00 | 2.00 × 10–02 | 0.3 | 2 | 1.7 | –1.12 | –49.7 | (34) | Figure 1 |
| Apo lipoprotein A1 | 122 | 7.4 | GndHCl | 0.952 | 1.05 | 5.00 × 10–03 | 0.6 | 1.7 | 1.1 | –0.61 | –32.8 | (35) | Figure 7 |
| Apo lipoprotein A1 | 122 | 7.4 | GndHCl | 0.95 | 1.05 | 3.00 × 10–02 | 0.3 | 2.3 | 2 | –1.21 | –55.9 | (36) | |
| lysozyme | 129 | 7 | GndHCl | 0.245 | 4.08 | 2.00 × 10–03 | 2.8 | 5.8 | 3 | –0.431 | –24.6 | (16) | Figure 1 |
| lysozyme | 129 | 2 | GndHCl | 0.42 | 2.38 | 2.00 × 10–02 | 0.8 | 4.6 | 3.8 | –1.03 | –49.6 | (37) | Figure 2A |
| lysozyme | 129 | 2.5 | GndHCl | 0.48 | 2.08 | 5.00 × 10–03 | 1.1 | 3.2 | 2.1 | –0.632 | –31.1 | (37) | Figure 1 |
| PMS-Ct | 145 | 4 | GndHCl | 0.52 | 1.92 | 1.00 × 10–03 | 1.1 | 2.5 | 1.4 | –0.486 | –22.7 | (38) | Figure 4 |
| PMS-Ct | 241 | 4 | GndHCl | 0.513 | 1.95 | 4.00 × 10–03 | 1.1 | 2.7 | 1.6 | –0.531 | –45.3 | (38) | Figure 4 |
| average | 0.551 | 2.22 | 0.012 | 2.931 | -0.90 | ||||||||
| STDV | 0.256 | 0.93 | 0.0096 | 1.659 | 0.43 | ||||||||
| Ac-tyr-(ala-glu-ala-ala-lys-ala)5-phe-NH2 | 32 | 7 | urea | 0.25 | 4.00 | 8.00 × 10–02 | (6) | Figure 1 | |||||
| Ac-tyr-(ala-glu-ala-ala-lys-ala)8-phe-NH2 | 50 | 7.0 | urea | 0.2 | 5.00 | 1.00 × 10–02 | 2.2 | 9.8 | 7.6 | –0.884 | –18.1 | (39) | Figure 1273 K |
| HPr protein | 85 | 7 | urea | 0.215 | 4.65 | 8.00 × 10–03 | 2 | 8 | 6 | –0.793 | –26.2 | (40) | Figure 1B288 K |
| cytochrome c | 106 | 5 | urea | 0.2 | 5.00 | 4.00 × 10–03 | 2.9 | 7.7 | 4.8 | –0.578 | –25.1 | (3) | |
| cytochrome c | 106 | 7 | urea | 0.155 | 6.46 | 4.00 × 10–03 | 3.6 | 9.5 | 5.9 | –0.574 | –22.5 | (3) | Figure 5 |
| P22 1-domaiin | 123 | 6 | urea | 0.3 | 3.33 | 9.00 × 10–03 | 1.5 | 5.6 | 4.1 | –0.753 | –35.18 | (41) | Figure 2 |
| lysozyme | 129 | 2 | urea | 0.303 | 3.30 | 2.00 × 10–02 | 2.2 | 6.4 | 4.2 | –0.632 | –47.9 | (37) | Figure 2B |
| lysozyme | 129 | 5.5 | urea | 0.15 | 6.67 | 2.00 × 10–03 | 4.5 | 9.3 | 4.8 | –0.43 | –23.3 | (16) | Figure 1 |
| anti-EF-R mab | 1600 | 7 | urea | 0.18 | 5.56 | 2.00 × 10–02 | 2 | 8.3 | 6.3 | –0.85 | –407 | (3) | Figure 2 |
| average | 0.22 | 4.80 | 1.74 × 10–02 | 5.46 | -0.69 | ||||||||
| STDV | 0.05 | 1.15 | 0.0229 | 1.12 | 0.15 | ||||||||
| lysozyme | 129 | SDS | 230.00 | 0.00 | 7.00 × 10–02 | 0.001 | 0.012 | 0.011 | –1.47 | –77.7 | (18) | Table 1 298 K |