Figure 1. The serotonin transporter C‐terminus harbors a potential MAD2 interaction motif (MIM). MAD2 protein expression in dorsal raphe neurons.

1. Clustal Omega alignment of the SERT C‐terminus with previously described MAD2‐interacting proteins. The putative SERT‐MIM and previously described MIMs are highlighted by blue boxes; (*)—fully conserved residue; (:)—residues of strongly similar properties; (.)—residues of weakly similar properties.
2. Cryosections were subjected to immunofluorescence microscopy using appropriate excitation wavelengths and emission filters. White boxes indicate the magnified area in the lower panel. All images were taken as “multiple image alignments.” Scale bars represent 100 and 25 μm in the upper and lower panel, respectively.
3. Whole‐brain and midbrain lysates of adult mice were prepared as outlined under “Materials and Methods.” Total protein (20 μg) was immunoblotted for MAD2 or α‐Tubulin and compared to HEK‐293 cell lysates.
4. Z‐stacks of individual TPH⁺/MAD2⁺ positive neurons were generated by confocal microscopy. Intensity profiles of an average projection were generated using ImageJ/Plot Profile software. Scale bar represents 20 μm.

Source data are available online for this figure.