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. 2023 Aug 2;24(10):e53408. doi: 10.15252/embr.202153408

Figure EV2. Association of full‐length transporters with BubR1, β2‐adaptin, and p31comet correlates with MAD2 association.

Figure EV2

  • A
    Densitometric signals in Fig 3C/co‐IP:YFP were quantified using ImageJ and normalized to their respective transporter signal intensities; error bars represent SEM. Data derive from seven (MAD2), six (BubR1), seven (p31comet), six (β2‐adaptin), and six (HSP70) independent biological replicates.
  • B–E
    Scatter plots for the co‐IP of indicated proteins (y‐axis) against the co‐IP of MAD2 (x‐axis) (cf. Fig 3C); r 2 indicates the squared linear correlation coefficient; ***P < 0.001, ****P < 0.0001, ns—not significant. Data derive from six (BubR1), seven (p31comet), six (β2‐adaptin), and six (HSP70) independent biological replicates.
  • F, G
    HEK‐293 cells were transfected with the indicated siRNAs and cell lysates were used for GST pull‐down experiments using the indicated GST proteins. One‐way ANOVA with Dunnett's multiple post hoc comparison; **P < 0.01, ***P < 0.001; error bars represent SD of three independent biological replicates.