Reagent/resource | Reference or source | Identifier or catalog number |
---|---|---|
Chemicals, enzymes, and other reagents | ||
Oligomycin | Sigma‐Aldrich | Cat# 75351 |
Rotenone | Sigma‐Aldrich | Cat# R8875 |
Antimycin A | Sigma‐Aldrich | Cat# A8674 |
FCCP | Sigma‐Aldrich | Cat# C2920 |
Glucose | Sigma‐Aldrich | Cat# G8769 |
Sodium Pyruvate | Sigma‐Aldrich | Cat# P5280 |
l‐Glutamine | Sigma‐Aldrich | Cat# G3126 |
HEPES | Sigma‐Aldrich | Cat# 83264 |
DMEM Powder | Sigma‐Aldrich | Cat# 5030 |
Sodium Chloride | Sigma‐Aldrich | Cat# S7653 |
Phenol Red | Sigma‐Aldrich | Cat# P0290 |
HCl | Sigma‐Aldrich | Cat# 2104 |
H2SO4 | Sigma‐Aldrich | Cat# 1.60315 |
Water suitable for cell culture | Sigma‐Aldrich | Cat# W3500 |
Software | ||
Agilent Seahorse XF Wave | Agilent | https://www.agilent.com/en/product/cell‐analysis/real‐time‐cell‐metabolic‐analysis/xf‐software/seahorse‐wave‐pro‐software‐2007523 |
GraphPad Prism | GraphPad | https://www.graphpad.com/ |
Excel | Microsoft | https://www.microsoft.com/en‐us/microsoft‐365/excel |
Other | ||
Seahorse XFe96 Analyzer | Agilent | https://www.agilent.com/en/product/cell‐analysis/real‐time‐cell‐metabolic‐analysis/xf‐analyzers/seahorse‐xfe96‐analyzer‐740879 |
Seahorse XFe96 FluxPak | Agilent | 102416‐100 |