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. 2023 Aug 7;24(10):e56380. doi: 10.15252/embr.202256380
Reagent/resource Reference or source Identifier or catalog number
Chemicals, enzymes, and other reagents
Oligomycin Sigma‐Aldrich Cat# 75351
Rotenone Sigma‐Aldrich Cat# R8875
Antimycin A Sigma‐Aldrich Cat# A8674
FCCP Sigma‐Aldrich Cat# C2920
Glucose Sigma‐Aldrich Cat# G8769
Sodium Pyruvate Sigma‐Aldrich Cat# P5280
l‐Glutamine Sigma‐Aldrich Cat# G3126
HEPES Sigma‐Aldrich Cat# 83264
DMEM Powder Sigma‐Aldrich Cat# 5030
Sodium Chloride Sigma‐Aldrich Cat# S7653
Phenol Red Sigma‐Aldrich Cat# P0290
HCl Sigma‐Aldrich Cat# 2104
H2SO4 Sigma‐Aldrich Cat# 1.60315
Water suitable for cell culture Sigma‐Aldrich Cat# W3500
Software
Agilent Seahorse XF Wave Agilent https://www.agilent.com/en/product/cell‐analysis/real‐time‐cell‐metabolic‐analysis/xf‐software/seahorse‐wave‐pro‐software‐2007523
GraphPad Prism GraphPad https://www.graphpad.com/
Excel Microsoft https://www.microsoft.com/en‐us/microsoft‐365/excel
Other
Seahorse XFe96 Analyzer Agilent https://www.agilent.com/en/product/cell‐analysis/real‐time‐cell‐metabolic‐analysis/xf‐analyzers/seahorse‐xfe96‐analyzer‐740879
Seahorse XFe96 FluxPak Agilent 102416‐100