Table 3.
PCR-array layout for gene expression profile in the intestine of gilthead seabream fed experimental diets.
| Function | Gene | Symbol | GenBank |
|---|---|---|---|
| Epithelial integrity | Proliferating cell nuclear antigen | pcna | KF857335 |
| Transcription factor HES-1-B | hes1-b | KF857344 | |
| Krüppel-like factor 4 | klf4 | KF857346 | |
| Claudin-12 | cldn12 | KF861992 | |
| Claudin-15 | cldn15 | KF861993 | |
| Cadherin-1 | cdh1 | KF861995 | |
| Cadherin-17 | cdh17 | KF861996 | |
| Tight junction protein ZO-1 | tjp1 | KF861994 | |
| Desmoplakin | dsp | KF861999 | |
| Gap junction Cx32.2 protein | cx32.2 | KF862000 | |
| Coxsackievirus and adenovirus receptor homolog | cxadr | KF861998 | |
| Nutrient transport | Intestinal-type alkaline phosphatase | alpi | KF857309 |
| Liver type fatty acid-binding protein | fabp1 | KF857311 | |
| Intestinal fatty acid-binding protein | fabp2 | KF857310 | |
| Ileal fatty acid-binding protein | fabp6 | KF857312 | |
| Mucus production | Mucin 2 | muc2 | JQ277710 |
| Mucin 13 | muc13 | JQ277713 | |
| Interleukins | Tumor necrosis factor-alpha | tnf-α | AJ413189 |
| Interleukin-1 beta | il-1β | AJ419178 | |
| Interleukin-6 | il-6 | EU244588 | |
| Interleukin-7 | il-7 | JX976618 | |
| Interleukin-8 | il-8 | JX976619 | |
| Interleukin-10 | il-10 | JX976621 | |
| Interleukin-12 subunit beta | il-12β | JX976624 | |
| Interleukin-15 | il-15 | JX976625 | |
| Interleukin-34 | il-34 | JX976629 | |
| Cell markers | Cluster of differentiation 4-1 | cd4-1 | AM489485 |
| Cluster of differentiation 8 beta | cd8b | KX231275 | |
| C–C chemokine receptor type 3 | ccr3 | KF857317 | |
| C–C chemokine receptor type 9 | ccr9 | KF857318 | |
| C–C chemokine receptor type 11 | ccr11 | KF857319 | |
| C–C chemokine CK8/C–C motif chemokine 20 | ck8/ccl20 | GU181393 | |
| Macrophage colony-stimulating factor 1 receptor 1 | csf1r1 | AM050293 | |
| Ig production | Immunoglobulin M | igm | JQ811851 |
| Immunoglobulin T membrane-bound form | igt-m | KX599201 | |
| Pattern recognition | Galectin-1 | lgals1 | KF862003 |
| receptors (PRRs) | Galectin-8 | lgals8 | KF862004 |
| Toll-like receptor 2 | tlr2 | KF857323 | |
| Toll-like receptor 5 | tlr5 | KF857324 | |
| Toll-like receptor 9 | tlr9 | AY751797 | |
| CD209 antigen-like protein D | cd209d | KF857327 | |
| CD302 antigen | cd302 | KF857328 | |
| Macrophage mannose receptor 1 | mrc1 | KF857326 | |
| Fucolectin | fcl | KF857331 |
Specific primer sequences for marker genes of intestine are listed in Supplementary Table S3 .