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. 2023 Oct 3;10(10):ENEURO.0089-23.2023. doi: 10.1523/ENEURO.0089-23.2023

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Copyright © 2023 Hernández-Vivanco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — PKA-induced iLTP is observed in GABA synapses originated in SST but not in PV INs. A, Schematic of experimental procedure. B, Representative traces of normalized oeIPSCs recorded in GCs from PV-Cre and SST-Cre mice infected with AAV-DIO-ChETA-EYFP. The blue light (490 nm) pulse used to elicit oeIPSCs is represented with a vertical blue bar. Scale bar, 10 ms. C, Representative images of Cre-dependent ChETA expression (cyan) and biocytin-filled GC (green) in the DG of slices prepared from PV-Cre and SST-Cre mice. Scale bar, 25 μm. D, Representative traces of oeIPSCs before (Bsl) and after FSK bath application in GCs from PV-Cre (PV_GC) and SST-Cre (SST_GC) mice. Calibration: 50 ms; 30 pA (SST-Cre) and 50 pA (PV-Cre). The time course of FSK effects on normalized oeIPSC amplitude in both conditions is shown in the right graph. Open symbols correspond to FSK application in the presence of PKA inhibitor H89 in experiments performed in SST-Cre mice (SST_GC+H89). E, Parts of whole graphs represent the proportion of recorded GCs showing significant increase of eIPSC amplitude after FSK application in PV-Cre and SST-Cre mice in the absence and presence of PKA inhibitor H89. Two-sided Fisher’s exact test; SST_GC versus PV_GC, p = 0.002; SST_GC versus SST_GC+H89, p = 0.0007; PV-Cre, n = 12 GCs; SST-Cre, n = 10 GCs; SST-Cre + H89, n = 8 GCs. F, Population data of FSK effects on normalized oeIPSC amplitude before (Bsl) and after FSK application in GCs from PV-Cre and SST-Cre mice. Two-way ANOVA; treatment/genotype: F_(2,27) = 20.64, p < 0.0001; Bonferroni’s multiple-comparisons test; PV-Cre Bsl versus FSK, p = 0.99; SST-Cre Bsl versus FSK, p < 0.0001; SST-Cre+H89 Bsl versus FSK, p = 0.71; PV-Cre mice, n = 12 GCs; SST-Cre mice, n = 10; SST-Cre+H89, n = 8 GCs. *p < 0.05; n.s., nonsignificant. Dots and vertical bars in D, and horizontal and vertical bars in F represent the mean ± SEM. Dots in F represent individual measurements.