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. Author manuscript; available in PMC: 2023 Oct 9.
Published in final edited form as: Science. 2023 Jul 13;381(6658):653–660. doi: 10.1126/science.adh3694

Fig. 1. A genome-wide CRISPR-Cas9 screen uncovers an essential function for 9-1-1 and RHNO1 in cells lacking BRCA2 and LIG4.

Fig. 1.

(A) Schematic of the three major DSB repair pathways in mammalian cells. (B) Western blot analysis of LIG4 and p53 in clonally derived BRCA2−/− LIG4−/− TP53−/− DLD1 cells (TKO). The asterisk indicates clones used in the screen. (C) Schematic of the dropout CRISPR/Cas9 screen to identify synthetic lethal interactions. (D) Genome-wide CRISPR-Cas9 screen result in TKO cells. Genes with a Bayes factor (BF) score >5 (intersection and x- and y-axes) were considered essential. (E) BF scores for the indicated genes. (F) Growth curve of TKO and TP53−/− cells treated with the indicated sgRNAs. Data are mean ± s.d. of three independent experiments normalized to time point zero (one day after seeding) and a control sgRNA (sgIL25).