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Inhibition of cholesterol synthesis reduces C. innocuum-induced NOD2 expression in lipid rafts. HT-29 cells were pretreated with simvastatin (100 μM) for 1 h prior to C. innocuum infection. Cells were probed with Hoechst 33,342 (blue), anti-NOD2 (green), and CTx-B (red), followed by confocal microscopy analysis. Bars, 5 μm.
