Experimental result	Optimized αSyn-SAA result
Three positive replicates	αSyn-SAA positive
Zero or one positive replicate	αSyn-SAA negative
Two positive wells and average $F_{\text{max}}<5,000$ RFU	αSyn-SAA negative
Two positive wells and coefficient of variation of $F_{\text{max}}>110$	αSyn-SAA negative
Two positive replicates	αSyn-SAA inconclusive (re-test recommended)