Skip to main content
. Author manuscript; available in PMC: 2023 Oct 9.
Published in final edited form as: Sci Signal. 2023 Mar 14;16(776):eabq0837. doi: 10.1126/scisignal.abq0837

Fig. 1. Arsenite induces necroptosis mediated by ZBP1.

Fig. 1.

(A) Viability assay in mouse IFNα (IFN)-primed and arsenite (Ars)-treated L929 cells as measured by SYTOX dye exclusion. N=3 sets of cells per group. See table S1 for the complete list of p-values. (B) Schematic of RIPK3 activation and execution of necroptosis. (C) Phosphorylation of MLKL (pMLKL) in L929 cells. N=4 independent experiments. (D) Trimerization (3x) of MLKL in L929 cells. TNFα+zVAD (T+Z) treatment was visualized on the same blot but with a shorter exposure time. N=3 independent experiments. (E) Viability assay in ZBP1 KO L929 cells as measured by SYTOX dye exclusion. N=3 sets of cells per group. (F) Phosphorylation and trimerization of MLKL in ZBP1 KO L929 cells. N=3 independent experiments. (G) Viability assay in primary MEFs as measured by propidium iodide dye exclusion. N=5 sets of cells per group. See table S2 for complete list of p-values. (H) Phosphorylation and trimerization of MLKL in primary MEFs. N=2 independent experiments. (I) Phosphorylation of MLKL in primary ZBP1 KO MEFs. N=2 independent experiments. Quantified data are presented as means ± SD. ***P < 0.001.