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. Author manuscript; available in PMC: 2023 Oct 9.
Published in final edited form as: Sci Signal. 2023 Mar 14;16(776):eabq0837. doi: 10.1126/scisignal.abq0837

Fig. 2. Arsenite-induced necroptosis depends on formation of stress granules.

Fig. 2.

(A and B) Stress granule formation assessed by immunofluorescence analysis for G3BP1 in MEFs 3 hours after arsenite (Ars) treatment (A). Quantification of stress granule formation (B). N=3 sets of cells per group. Scale bar, 10 μm. (C and D) Viability assay in MEFs primed with mouse IFNα (IFN) and treated with arsenite as measured by propidium iodide dye exclusion (C). Quantification of cell viability (D). N=6 sets of cells per group. Scale bar, 100 μm. (E) Phosphorylation of MLKL (pMLKL) in MEFs and L929 cells. N=3 independent experiments. (F) Trimerization (3x) of MLKL in MEFs. N=3 independent experiments. (G and H) Viability assay in U2OS cells transfected with the indicated plasmids as measured by propidium iodide dye exclusion at 14 hours following arsenite treatment (G). Quantification of cell viability (H). N=3 sets of cells per group. Scale bar, 20 μm. (I) Stress granule formation assessed by immunofluorescence analysis for TIAR in U2OS cells at 2 hours following arsenite treatment. Cells were transfected with the indicated plasmids and treated with arsenite 24 hours later. Red arrows indicate cells with stress granules. N=3 sets of cells per group. Scale bars, 20 μm. (J) Viability assay in U2OS cells measured as in (G). Cells were transfected with the indicated plasmids. Quantified data are presented as means ± SD. ***P < 0.001.