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. Author manuscript; available in PMC: 2023 Oct 9.
Published in final edited form as: Sci Signal. 2023 Mar 14;16(776):eabq0837. doi: 10.1126/scisignal.abq0837

Fig. 3. Canonical stress granules are required for necroptosis activated by arsenite.

Fig. 3.

(A) Schematic of the ISR leading to stress granule formation. Inhibitors of the pathway, integrated stress response inhibitor (ISRIB) and cycloheximide (CHX), are indicated. (B and C) Stress granule formation assessed by immunofluorescence analysis for G3BP1 in CHX- and ISRIB-treated L929 cells at 1 hour post-arsenite (Ars) treatment, with or without mouse IFNα (IFN) priming (B). Quantification of stress granule formation (C). N=6 sets of cells per group. Scale bar, 20 μm. (D and E) Viability assay in L929 cells measured by propidium iodide dye exclusion (D). Quantification of cell viability (E). N=3 sets of cells per group. Scale bar, 100 μm. (F) Phosphorylation (pMLKL) and trimerization (3x) of MLKL in L929 cells. N=2 independent experiments. Quantified data are represented as means ± SD. ***P < 0.001.