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. Author manuscript; available in PMC: 2023 Oct 9.
Published in final edited form as: Sci Signal. 2023 Mar 14;16(776):eabq0837. doi: 10.1126/scisignal.abq0837

Fig. 4. ZBP1 localizes to stress granules during arsenite-induced necroptosis.

Fig. 4.

(A and D) Protein localization assessed by immunofluorescence analysis for G3BP1 and ZBP1 in unprimed or mouse IFNα (IFN)-primed (A) or IFN-primed cycloheximide (CHX) or integrated stress response inhibitor (ISRIB)-treated (D) L929 cells at 1 hour following arsenite (Ars) treatment. N=6 sets of cells per group. Scale bars, 30 μm in (A) and 50 μm in (D). (B) Quantification of the formation of ZBP1-containing stress granules in IFN-primed L929 cells from assays in (A) and (D). (C) Quantification of colocalization of stress granules containing G3BP1 and ZBP1 in cells shown in (A). (E to G) Protein localization assessed by immunofluorescence analysis for ZBP1 and G3BP1 in IFN-primed WT MEF or Tia1−/− MEFs at 3 hours following arsenite treatment (E). Quantification of ZBP1-containing stress granules (F) and of colocalization of G3BP1 and ZBP1 (G). N=3 sets of cells per group. Scale bar, 50 μm. Quantified data are presented as means ± SD. ***P < 0.001.