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. 2023 Sep 22;8(18):e162893. doi: 10.1172/jci.insight.162893

Figure 9. NUDT21 functions as a tumor suppressor mainly depending on MORC2 downregulation.

Figure 9

(A) Immunoblotting was performed to evaluate the expression of Flag-NUDT21 and Flag-MORC2 in Caki-1 and A498 cells transfected with empty Flag vector or Flag-NUDT21 plasmid with or without MORC2 recovery. (B) CCK8 assays were performed to evaluate the proliferation rate of Caki-1 and A498 cells transfected with empty Flag vector or Flag-NUDT21 with or without MORC2 recovery (n = 3). (C) Colony formation assays were performed and quantitatively analyzed to evaluate the clonogenicity of Caki-1 and A498 cells transfected with empty Flag vector or Flag-NUDT21 with or without MORC2 recovery (n = 3). (D and E) In vivo xenograft tumor formation experiment was performed (D) and quantitatively analyzed (E) with control Caki-1 and NUDT21-stably expressed Caki-1 cells with or without MORC2 recovery (n = 5 per group). (F) Immunoblotting was performed to evaluate expression of NUDT21 and MORC2 in Caki-1 and A498 cells transfected with control siRNA or NUDT21-specific siRNA with or without MORC2 silencing. (G) CCK8 assays were performed to evaluate the proliferation rate of Caki-1 and A498 cells transfected with control siRNA or NUDT21-specific siRNA with or without MORC2 silencing (n = 3). (H) Colony formation assays were performed and quantitatively analyzed to evaluate the clonogenicity of Caki-1 and A498 cells transfected with control siRNA or NUDT21-specific siRNA with or without MORC2 silencing (n = 3). (I) Prognosis of patients with KIRC with high NUDT21 expression and low MORC2 expression or low NUDT21 expression and high MORC2 expression was analyzed with Xiantao database (www.xiantaozi.com) depending on TCGA data. All data represent the mean ± SD. Two-tailed t test analyses were performed. *P < 0.05; **P < 0.01; ***P < 0.001.