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. 2023 Sep 22;8(18):e170739. doi: 10.1172/jci.insight.170739

Figure 5. Biopsies of DD exhibit mislocalization of cadherins, loss of keratin 10, and elevated ERK activation.

Figure 5

(A) H&E-stained cross sections of punch biopsies from skin of control donors versus patients with DD, which demonstrate loss of keratinocyte cohesion (acantholysis) and aberrant cornification (dyskeratosis) with retention of nuclei in cornified layers (magnified in inset; original magnification = 40×); scale bar = 100 μm. (B) Quantification of immunostaining of KRT10 (relative to Hoechst) and p-ERK (relative to total ERK) in cross sections of control skin versus in lesional or nonlesional portions of DD biopsies; graph depicts mean ± SD from N ≥ 20 nonoverlapping hpf per group from 5 control and 5 DD patients; control mean normalized to 1; P values from 1-way ANOVA with Dunnett’s adjustment for multiple comparisons. (C) Immunostaining of KRT10, (D) DSG1, and (E) p-ERK in tissue cross sections from patient biopsies; images from 2 control and 2 DD patients representative of 5 patients in each group; scale bar = 50 μm; dashed line marks bottom of epidermis.