Figure 6. Inhibition of polyamine biosynthesis augments Trm cell generation in BM.

(A) Schematic of the experimental design of the long-term BM CD8⁺ T cell ACT vaccination model. Two months following transplant of CD45.1⁺CD8⁺ OT-I T cells (stimulated with SIINFEKL and IL-2 ± 5 mM DFMO for 2 days prior to transplant) and 1 month following vaccination with SIINFEKL peptide + LPS, BM cells of CD45.2⁺ recipient mice were assessed for percentage of CD45.1⁺ OT-I. (B and C) CD8⁺ T cells and CD8⁺BCL2⁺ T cells are shown. (D) MFI of CD69 in CD45.1⁺ OT-I T cells. (E–G) Percentages of Ly6C⁺, CD69⁺CD103⁺, and CD69⁺CXCR6⁺ of CD45.1⁺CD8⁺ OT-I T cells in the BM of CD45.2⁺ recipient mice. (H) Percentage of CD45.1⁺CD8⁺ OT-I T cells in the spleens of CD45.2⁺ recipient mice. (I and J) MFI of IFN-γ and TNF-α in CD45.1⁺ CD8⁺ OT-I BM T cells. Unpaired t test was used. Each dot represents a biological replicate, and all data are shown as mean ± SEM (n = 5). *P < 0.05; **P < 0.01.