Figure S2. Myofiber-specific ablation of Fn14 does not affect regenerative myogenesis.

(A) Fn14^fl/fl mice were crossed with muscle creatine kinase-Cre mice to generate muscle-specific Fn14-knockout (Fn14^mKO) mice and littermate Fn14^fl/fl mice. Adapted from “Generation of Cre-LoxP-mediated Conditional Knockout Mice,” by BioRender.com (2023). (B) Average body weight of 8-wk-old littermate Fn14^fl/fl and Fn14^mKO mice. n = 3 per group. (C) Wet weight of uninjured and 5d-injured TA muscles normalized by body weight in Fn14^fl/fl and Fn14^mKO mice. n = 3–6 per group. (D) Representative photomicrographs of hematoxylin-and-eosin (H&E)–stained sections of uninjured and 5d-injured TA muscles of Fn14^fl/fl and Fn14^mKO mice. Scale bar: 50 μm. (E) Quantification of the average myofiber cross-sectional area with centralized nuclei. n = 3–6 in each group. (F) Percentage of myofibers with two or more centrally located nuclei in 5d-injured TA muscle sections of Fn14^fl/fl and Fn14^mKO mice. n = 3–5 per group. (G) Representative photomicrographs of 5d-injured TA muscle sections of Fn14^fl/fl and Fn14^mKO mice after immunostaining for eMyHC (red) and laminin (green) proteins. Nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. Data are presented as the mean ± SEM by an unpaired t test or by a two-way ANOVA followed by Tukey’s multiple-comparison test. *P ≤ 0.05, values significantly different from the corresponding uninjured muscle.