In Vivo Therapeutic Effect of Osteoarthritis. (a) A flow diagram showing the time of intra-articular injection. (b) H&E, Safranin O/fast green, Toluidine Blue staining of mice knee joints on 8th week. Scale bar = 200 μm. (c) OARSI grade of the knee joints of DMM mice on 8th week. (d) Histological scores of the knee joints of DMM mice on 8th week. (E) Mankin's scores of the knee joints of DMM mice on 8th week. (f) Immunofluorescence analysis of ADAMTS4, ACAN, COMP and Col2a1 in knee joints of DMM mice on 8th week. Scale bar = 200 μm. Scale bar = 200 μm *p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)