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Purification of the TEM-60 β-lactamase produced by P. stuartii VR-1
| Step | Total protein (mg) | Specific activity (U/mg) | Total activity (U) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude lysatea | 260 | 2.6 | 676 | 1 | 100 |
| Sepharose Q-FF | 8.5 | 52.0 | 442 | 20 | 65 |
| Chromatofocusing | 0.55 | 305.0 | 195 | 117 | 29 |
The crude lysate was obtained from 2 liters of an overnight culture.
