TABLE 5.
Comparative analysis of catalytic efficiency between TEM-60 from P. stuartii VR-1 and some related TEM enzymes
| Antibiotics |
kcat/Km (μM−1 s−1)
|
|||
|---|---|---|---|---|
| TEM-60 | TEM-3a | TEM-18a | TEM-1a | |
| Benzylpenicillin | 2.8 | 9.5 | 69 | 84 |
| Carbenicillin | 0.5 | n.d.b | n.d. | n.d. |
| Ampicillin | 0.9 | 3.8 | 26 | 33 |
| Piperacillin | 0.9 | n.d. | n.d. | n.d. |
| Amoxicillin | 12 | n.d. | n.d. | n.d. |
| Cephaloridine | 0.53 | 1.1 | 2.1 | 2.2 |
| Cephalotin | 0.06 | 1.9 | 1.2 | 0.65 |
| Cefuroxime | 0.12 | 0.15 | 0.02 | 0.006 |
| Cefotaxime | 0.05 | 1.0 | 0.08 | 0.0015 |
| Desacetyl cefotaxime | 0.04 | n.d. | n.d. | n.d. |
| Ceftazidime | 0.2 | 0.04 | 0.008 | 0.0007 |
| Nitrocefin | 0.85 | 7.3 | 38 | 17 |
| Aztreonam | 0.16 | 0.2 | 0.014 | 0.007 |
| Carumonam | 0.01 | n.d. | n.d. | n.d. |
a
Data from reference 18.
b
n.d., not determined.