TABLE 6.

Kinetic parameters for the inactivation of the TEM-60 β-lactamase

β-lactam	K (μM)	k+2 (s−1)	k+3 (s−1)	k+2/K (M−1 s−1)
β-IP	8 · 10−2	2.1 · 10−2	n.d.b	2.6 · 105
Sulbactam	7 · 10−2	n.d.	n.d.	n.d.
Tazobactam	2 · 10−2	n.d.	4.2 · 10−4a	2.1 · 104
Clavulanate	0.7	n.d.	8.7 · 10−3	1.2 · 104

^aMeasured from the deacylation of the complex using 100 μM nitrocefin as substrate. 

^bn.d., not determined.