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. 2023 Aug 7;20(3):1011–1027. doi: 10.14245/ns.2346446.223

Fig. 4.

Fig. 4.

MiR-106b-5p regulated the expressions of autophagy and apoptosis proteins in vitro. (a) A qRT-PCR analysis determined the expression of miR-106b-5p in SH-SY5Y cells transfected with miR-106b-5p mimics/NC mimics and miR-106b-5p inhibitors/ NC inhibitors. (b–g) Representative Western blot (WB) bands and the corresponding quantification data of P62, Beclin-1, Caspase-3, Bax and, LC3 in SH-SY5Y cells; GAPDH was used as an endogenous control. Continuous characteristics were compared using Student t-tests, and all the results are shown as the mean±standard deviation (n=3/group). Further, all experiments were repeated at least thrice. qRT-PCR, quantitative real-time polymerase chain reaction; NC, negative control. *p<0.05. **p<0.01, as compared with the NC inhibitor group. †p<0.05. ††p<0.01, as compared with the NC mimics group.