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. 2023 Sep 21;55(10):1721–1734. doi: 10.1038/s41588-023-01504-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a,b, IF images (a) and quantification (b) of MCF10A WT and A3B KO cells stained with S9.6 (green) and DAPI (blue) (representative images; 5 μm scale; n = 3 independent experiments with >100 nuclei per condition; red bars represent mean ± s.e.m.; P value by Mann–Whitney test). c,d, S9.6 dot-blot analysis of MCF10A WT and A3B KO genomic DNA dilution series ± exogenous RNase H (RNH; representative images); parallel dsDNA dot blots provided a loading control (c). Quantification normalized to the most concentrated WT signal (representative experiment shown from four independent experiments; mean ± s.e.m.; P value by two-tailed unpaired t-test) (d). e,f, IF images (e) and quantification (f) of U2OS shCtrl and shA3B cells stained with S9.6 (green) and DAPI (blue; representative images; 5 μm scale; n = 3 independent experiments with >100 nuclei per condition; red bars represent mean ± s.e.m.; P value by Mann–Whitney test). g,h, S9.6 dot-blot analysis of a U2OS shCtrl and shA3B genomic DNA dilution series ± exogenous RNase H (RNH; representative images); parallel dsDNA dot blots provided a loading control (g). Quantification normalized to the most concentrated shCtrl signal (representative experiment shown from three independent experiments; mean ± s.e.m.; P value by two-tailed unpaired t-test) (h). i,j, IF images (i) and quantification (j) of MCF10A WT and A3B KO cells stained with S9.6 (green), DAPI (blue) and γ-H2AX (representative images; 5 μm scale; n = 3 independent experiments with >100 nuclei per condition; red bars represent mean ± s.e.m.; P value by Mann–Whitney test (left); P value by two-tailed unpaired t-test (right). k,l, IF images (k) and quantification (l) of U2OS shCtrl and shA3B cells stained with S9.6 (green), DAPI (blue) and γ-H2AX (representative images; 5 μm scale; n = 3 independent experiments with >100 nuclei per condition; red bars represent mean ± s.e.m.; P value by Mann–Whitney test (left); P value by two-tailed unpaired t-test (right).

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