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. 2023 Sep 21;55(10):1721–1734. doi: 10.1038/s41588-023-01504-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Schematic of the AP-MS workflow used to identify the cellular A3B interactome. A3B is shaded orange/green and cellular proteins are indicated by different shapes/colors. b-c, Anti-Flag immunoblot and Coomassie gel analysis of eGFP-SF and A3B-SF following affinity purification and prior to analysis by mass spectrometry (**, samples not pertaining to this manuscript; representative images; n = 6 independent experiments). d, DNA deaminase activity of eGFP-SF and A3B-SF following affinity purification (purified A3A was used as a positive control; **, samples not pertaining to this manuscript; representative images; n = 6 independent experiments). e, co-IP of indicated Flag-tagged interactors and HA-tagged A3B in 293 T cells (representative data from n = 2 independent experiments). Upper immunoblots show the indicated proteins in whole cell lysates (input), and lower immunoblots show the Flag-immunoprecipitated samples (elution). kDa markers are shown the left of each blot and the primary antibody used for detection is shown to the right. f, co-IP of indicated Flag-tagged interactors and eGFP-tagged A3B or eGFP-tagged Mut2 from 293 T cells (representative data from n = 2 independent experiments). Upper immunoblots show the indicated proteins in whole cell lysates (inputs), and lower immunoblots show the anti-Flag immunoprecipitated samples (elutions). kDa markers are shown to the left of each blot and the primary antibody used for detection is shown to the right.

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