Table 2.
List of transcripts expressed in adult female ticks fed on vaccinated vs control cattle hosts
| General pathway | Contig on array | Description | Putative localization | logFC | Adj. P * |
|---|---|---|---|---|---|
| Energy transport and metabolism | T0000903 | Putative facilitated glucose transporter, Solute carrier family 2 | Extracellular. Cell membrane, multi-pass membrane protein | 2.58 | 5.30E−05 |
| Contig2278 | Putative glucose-6- phosphatase | Intracellular. Endoplasmic reticulum, multi-pass membrane protein | 3.93 | 6.50E−05 | |
| Growth and development | TC16526 | Putative granulin | Extracellular. Secreted protein | 3.20 | 8.21E−04 |
| Innate immunity | TC23502 | Microplusin | Extracellular. Secreted protein | − 3.82 | 8.20E−04 |
| CK189663 | Putative ixoderin, Techylectin-5A-like | Extracellular. Secreted protein | − 4.00 | 2.58E−04 | |
| TC22409 | Putative ixoderin, Techylectin-5A-like | Extracellular. Secreted protein | − 4.73 | 6.22E−05 | |
| TC19369 | Putative ixoderin, Techylectin-5A-like | Extracellular. Secreted protein | − 3.19 | 3.60E−04 | |
| AA257910 | Putative tubulin alpha-1A chain | Intracellular. Cytoplasm, cytoskeleton | 2.83 | 5.72E−04 | |
| CV444905 | Cystatin-L2-like cysteine peptidase inhibitor | Intracellular. Cytoplasm | − 2.49 | 1.50E−03 | |
| Oxidative stress | T0004546 | Putative tubulin alpha-1C chain | Intracellular. Cytoplasm, cytoskeleton | 2.45 | 4.47E−04 |
| F1-2-A_Clone_20 | Putative heat shock protein HSP 90-beta | Intracellular and extracellular | 1.77 | 1.57E−03 | |
| T0000364 | Putative heat shock protein 105 kDa | Intracellular. Cytoplasm | 1.60 | 2.00E−03 | |
| CK184995 | Putative ubiquitin-like modifier-activating enzyme | Intracellular. Cytoplasm (and nucleus) | − 2.76 | 3.16E−04 | |
| TC24883 | Putative hypoxia-inducible prolyl hydroxylase 2 | Intracellular. Mainly cytoplasmic | − 3.35 | 2.91E−04 | |
| TC17253 | Putative glutathione S-transferase 1 | Intracellular. Cytoplasm | − 3.83 | 6.11E−04 | |
| TC17897 | Putative glutathione S-transferase Mu 2 | Intracellular. Cytoplasm | − 3.42 | 7.80E−04 | |
| TC17316 | Putative glutathione S-transferase Mu 2 | Intracellular. Cytoplasm | 1.72 | 1.08E−03 | |
| TC16153 | Putative methanethiol oxidase, Selenium-binding protein 1-A (alt) | Intracellular. Cytosol and nucleus as peripheral membrane protein | − 2.85 | 1.84E−04 | |
| TC18726 | Putative O-glucosyltransferase rumi homolog | Extracellular. Endoplasmic reticulum, secreted protein | − 2.29 | 6.38E−04 | |
| TC23300 | Putative E3 ubiquitin-protein ligase NEURL1B-like | Intracellular. Cytoplasm | − 3.66 | 1.59E−04 | |
| NP1774071 | Ferritin | Intracellular. Cytoplasm | − 3.50 | 5.30E−05 | |
| Digestion and embryogenesis | TC17290 | Legumain-like | Intracellular. Cytoplasm | − 2.70 | 9.66E−05 |
| T0010166 | Putative cathepsin D-like | Intracellular. Lysosomal membrane | − 2.18 | 1.20E−03 | |
| TC16620 | Putative cathepsin D-like | Intracellular. Lysosomal membrane | − 4.17 | 3.43E−05 | |
| CV445464 | Putative cathepsin D-like | Intracellular. Lysosomal membrane | − 3.58 | 6.58E−05 | |
| TC15655 | Cathepsin L | Intracellular. Lysosomal | 1.90 | 2.31E−03 | |
| Contig1628 | Cathepsin L | Intracellular. Lysosomal | 2.01 | 7.67E−04 | |
| Contig1379 | Cathepsin C | Intracellular. Lysosomal | 1.63 | 6.24E−03 | |
| TC23222 | Vitellogenin-2-like | Extracellular. Secreted protein | − 2.34 | 3.04E−04 | |
| TC21529 | Vitellogenin-2-like | Extracellular. Secreted protein | − 3.59 | 1.32E−05 | |
| TC21162 | Vitellin-degrading cysteine endopeptidase | Extracellular. Secreted protein | − 2.39 | 1.39E−04 | |
| T0005046 | Putative ecdysteroid receptor | Intracellular. Nucleus | 2.04 | 2.77E−04 | |
| TC20218 | Niemann Pick type C 1a | Extracellular. Plasma membrane | 1.91 | 3.54E−04 | |
| T0009333 | Palmitoyltransferase ZDHHC17-like | Intracellular. Golgi membrane | 0.91 | 1.93E−02 | |
| TC23621 | Serine protease inhibitor | Intracellular. Cytoplasm | − 5.57 | 3.02E−05 | |
| TC20102 | Serine protease inhibitor | Intracellular. Cytoplasm | − 4.70 | 9.75E−05 |
Indicated are transcripts that are significantly differentially expressed (P < 0.01, logFC ≥ 2 or ≤ − 2). Additional non-differentially expressed transcripts of interest are also indicated with functions within identified pathways
*Adjusted P < 0.01 and upper and lower 1% of logFC distribution